Background: Antibiotic resistance has become one of the major problems facing humanity. The need for new\nantimicrobials has been increased dramatically. Plants are considered as one of the most promising sources for new\nantimicrobials discovery. Despite relatively small area, Armenia has large diversity of flora with many endemic\nspecies. In Armenian folk medicine plant materials have been used to treat various microbial diseases since ancient\ntimes. The goal of our research was to evaluate antimicrobial efficiency of different parts of five wild plants species\nwhich are commonly used in Armenian traditional medicine.\nMethods: Plant crude extracts were obtained with maceration technique using five solvents separately: distilled\nwater, methanol, chloroform, acetone, and hexane. Agar well diffusion assay was used for initial evaluation of\nantimicrobial properties of plant materials against five bacterial and two yeast strains. Minimum inhibitory concentrations\nof the most active plant parts were determined by broth microdilution method.\nResults: Crude extracts of all five tested plants expressed antimicrobial activity against at least four test strains at\n500 Ã?¼g mlâË?â??1 concentration. Minimum inhibitory and bactericidal/fungicidal concentrations of selected plant parts were\ndetermined. Crude acetone and hexane extracts of Hypericum alpestre and acetone extract of Sanguisorba officinalis\ninhibited the growth of P. aeruginosa even at 64 Ã?¼g mlâË?â??1 concentration. Chloroform and acetone extracts of Sanguisorba\nofficinalis exhibited cidal activity against P. aeruginosa till 256 Ã?¼g mlâË?â??1. Acetone was the most effective solvent for\nsolubilizing antimicrobial compounds for almost all tested plant materials.\nConclusions: Thus, antimicrobial activity of some medicinal plants used in Armenian traditional medicine was evaluated.\nSome of the plants had rather low minimum bacteriostatic/bactericidal concentrations and therefore they have\nprospective for further more inclusive studies.
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